Differences in Mitochondrial Genome Organization of Cryptococcus Neoformans Strains |
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Authors: | Judit Litter Andrea Keszthelyi Zsuzsanna Hamari Ilona Pfeiffer Judit Kucsera |
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Institution: | (1) Department of Microbiology, Faculty of Sciences, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary |
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Abstract: | The organization of the mitochondrial genomes in two strains belonging in different varieties of Cryptococcus neoformans was analysed. Physical maps of the mtDNA of the IFM5844 (var. neoformans) and IFO410 (var. grubii) strains were constructed by using EcoRI and EcoRV restriction enzymes; functional maps were constructed by hybridization, cloning and sequencing. Most of the genes important
in the mitochondrial function (ND1, ND2, ND3, ND4, ND4L, ND5, ND6, ATP6, ATP9, COX1, COX2 and COB) and protein synthesis (SsrRNA and LsrRNA) were localized. We did not find any differences between the strains in the order of these genes. However, they differed
significantly in the sizes of the mtDNAs: 32.6 kb for IFM5844, and 24.1 kb for IFO410. This can be attributed to two large
regions of the mtDNA. In these regions, differences were found in the numbers of introns in COX1 (no intron in var. grubii, 5 introns in var. neoformans), COB (1 intron in var. grubii, 2 introns in var. neoformans), LsrRNA (no intron in var. grubii, 2 introns in var. neoformans), and ND5 (no intron in var. grubii, 1 intron in var. neoformans) genes. In several introns of the COB and COX1 genes LAGLIDADG motifs were found. Differences were also observed in the nucleotide sequences of some genes and in the sizes
and sequences of intergenic regions. The nucleotide sequences of the genes of the IFM and IFO strains were compared with those
of the H-99 and JEC 21 strains from the database. Surprisingly high similarities were found between the strains belonging
in var. grubii (IFO 410 and H-99) and var. neoformans (IFM 5844 and JEC 21). |
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Keywords: | Cryptococcus neoformans Intron LAGLIDADG motif Mitochondrial DNA Physical map |
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