成年和老年小鼠脑蛋白质组双向电泳图谱比较

使用双向电泳(2-DE)比较成年和老年小鼠脑蛋白质差异，从分子水平初步探索老年脑蛋白整体变化规律.以固相pH梯度等电聚焦为第一向，SDS-聚丙烯酰胺凝胶水平电泳(PAGE)为第二向进行2-DE.图象分析软件Imagemaster^® 2D Elite分析电泳图谱.重复性实验结果显示，同组样品在三次不同实验中所得蛋白质斑点数目的相对标准差（变异系数）为4.43%±0.25%；同一蛋白质斑点在三次实验中等电点、分子质量和蛋白质量的相对标准差分别为8.76%±5.14%, 13.00%±4.22%和10.84%±9.16%.成年和老年小鼠脑组织2-DE图谱分别获得996和1256个蛋白质斑点，其中8个蛋白质在老年脑组织中含量降低，20个蛋白质斑点含量增加.另至少有4个蛋白质斑点在老年脑组织中缺失，14个蛋白质点为老年脑特有. 以上差异点的发现为研究脑老化和退行性疾病机理提供了有益的线索.

Comparison of the Two-dimensional Electrophoresis Patterns Between Adult and Aged Murine Brains in Proteomatic Analysis

A wide variety alterations are common in brains of aged individuals. Proteomatic analysis is a new power tool to reveal such changes at protein level. The two-dimensional electrophoresis (2-DE) was employed to compare the global protein patterns between adult and aged murine brains. One hundred and fifty micrograms of murine brain protein extracted with 5 mol/L urea, 2 mol/L thiourea, 2% CHAPS plus 2% SB3-10 was run immobilized pH gradient (IPG) isoelectric focusing electrophoresis as the first dimension, and then horizontal SDS-PAGE as the second dimension. The relative standard deviations(coefficient of variation) for protein number, pI, molecular mass and volume of protein in three different experiments were 4.43%±0.25%, 8.76%±5.14%, 13.00%±4.22% and 10.84%±9.16%, respectively. Totally, 996 and 1 256 protein spots were obtained in adult and aged murine brain map, repectively, of which 8 spots increased and 20 spots decreased in quantity. Furthermore, 4 spots were missing and 14 new spots emerged in aged murine brain compared with adult mice. The differentially displayed proteins between adult and aged murine brain are useful for diagnosing brain degenerative disorders.