抗凝活性蛋白产生菌Termitomyces albuminosus的诱变育种

真菌Termitomyces albuminosusTA-SD能产生具有抗凝活性的蛋白质TA-P。本实验采用紫外诱变法对TA-SD菌株进行诱变育种,筛选高活性的突变株。出发菌株TA-SD紫外照射40s时,正突变率和抗凝活性提高的幅度最大,分别为33.3%和104.7%。N型突变株和放线菌酮抗性突变株的正突变率和活性提高的幅度均大于S型突变株和非抗性突变株。经筛选突变株SD-A-8的抗凝活性比出发菌株提高了2.3倍。该突变株传代稳定。

Mutation breeding of Termitomyces albuminosus producing anti-coagulation protein TA-P

The fungus,Termitomyces albuminosus TA-SD could produce anti-coagulation protein TA-P.In order to screening the mutants with higher activities,the mutation breeding of the strain TA-SD was carried out by ultraviolet mutagenesis.When the time of ultraviolet radiation was 40 seconds,the most positive mutation rate and anti-coagulation activity were reached,and it was 33.3% and 104.7% respectively.Both the positive mutation rate and anti-coagulation activity in the N-type mutants and cycloheximide-resistant mutants were higher than it in the S-type mutants and non-cycloheximide-resistant mutants.After screening the mutant SD-A-8 was obtained and its anti-coagulation activity was 2.3 times higher than the initial strain.The mutant was stable after the transfer of culture.