High-conductance K+ channels in guinea pig gallbladder epithelium] |
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Authors: | G Meyer |
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Institution: | Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano. |
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Abstract: | Since secretion of electrolytes may be regulated by membrane potential difference, ion channels were studied using patchclamp technique. We have identified, in cell-attached configuration, inward-rectifying channels: the zero-current potential corresponded to the K+ equilibrium potential calculated from intracellular K+ activity. Using inside-out configuration and symmetric 145 mM KCl salines, i/V curve was linear, channel conductance was about 170 pS and the reversal potential 0 mV. The channels were selective for K+ over Na+, N-methylglucamine and anions and were activated by membrane depolarization. |
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