MCF-7 breast carcinoma cells express ryanodine receptor type 1: functional characterization and subcellular localization |
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| Authors: | Carlos Saldaña Mauricio Díaz-Muñoz Anaid Antaramián Adriana González-Gallardo Pablo García-Solís Verónica Morales-Tlalpan |
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| Affiliation: | 1. Cellular and Molecular Neurobiology Department, Neurobiology Institute, Campus UNAM-Juriquilla, 76230, Querétaro, QRO, Mexico
2. Proteogenomic Unit, Neurobiology Institute, Campus UNAM-Juriquilla, 76230, Querétaro, QRO, Mexico
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| Abstract: | Breast carcinoma-derived MCF-7 cells are frequently used in biomedical research. However, few reports exist regarding the characterization of signaling mechanisms in these cancerous cells involved in intracellular Ca2+ dynamics. Consequently, the aim of these experiments was to characterize the ryanodine receptor/Ca2+ release channel (RyR) present in MCF-7 cells. Ryanodine (100 nM), cADPR (5 μM), and caffeine (10 mM) promoted cytoplasmic Ca2+ mobilization; in contrast, ryanodine at inhibitory concentration (100 μM) decreased the basal Ca2+ level. Fluorescent probes demonstrated that RyR is located mainly in endomembranes. Some degree of co-localization with inositol trisphosphate receptor (IP3R) was observed, whereas coincidence with thapsigargin-sensitive Ca2+-ATPase (SERCA) was more limited. Molecular cloning resulted in the detection exclusively of RyR isoform 1. For the first time, it is shown that MCF-7 cells express functional RyR. |
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