Molecular Cloning of cDNA to mRNA for a Cerebellar Spot 35 Protein |
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| Authors: | T Yamakuni R Kuwano S Odani N Miki† K Yamaguchi‡ Y Takahashi |
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| Institution: | Department of Neuropharmacology, Brain Research Institute, Tokyo, Japan;Department of Biochemistry, School of Medicine, Niigala University, Niigata, Tokyo, Japan;Department of Pharmacology, Cancer Research Institute, Kanazawa University, Kanazawa, Tokyo, Japan;Kyowa Hakko Company, Machida, Tokyo, Japan |
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| Abstract: | The nucleotide sequence of mRNA for rat cerebellar spot 35 protein, a Ca-binding protein, was determined from recombinant complementary DNA (cDNA) clones. The sequence was composed of 1,714 base pairs (bp) which included the 783 bp of the complete coding region, the 130 bp of the 5'-noncoding region, and the 801 bp of the 3'-noncoding region containing a polyadenylation signal. In addition, a polyadenylic acid poly(A)] tail was also found. Because the size of spot 35 mRNA was estimated to be about 1,900 bases by Northern blot analysis, the longest insert was verified to contain a nearly full-length cDNA sequence including the poly(A) tail. The amino acid sequence of the protein deduced from the nucleotide sequence contains 261 amino acids and at least five Ca-binding domains. There was a high homology in the amino acid sequences (79%) and the nucleotide sequences (77%) between spot 35 protein and chick intestinal Ca-binding protein (28K). |
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| Keywords: | cDNA Nucleotide sequence Cerebellum Pro tein Purkinje cell mRNA |
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