Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice |
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Authors: | Ohta Masaru Hayashi Yasuyuki Nakashima Asae Hamada Akira Tanaka Akira Nakamura Tatsunosuke Hayakawa Takahiko |
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Affiliation: | Plantech Research Institute, 1000 Kamoshida-cho, Aoba-ku, Yokohama, 227-0033, Kanagawa, Japan. |
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Abstract: | We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by non-stress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance. |
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Keywords: | Halophyte plants Sodium/proton antiporter Rice Salt tolerance Transgenic plants |
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