Fluorescence and photoelectron studies of the intercalative binding of benz(a)anthracene metabolite models to DNA |
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Authors: | M Shahbaz R G Harvey A S Prakash T R Boal I S Zegar P R LeBreton |
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Institution: | 1. European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, R.F.A. Germany;2. Laboratoire de Biochimie, Université de Neuchâtel, Ch. de Chantemerle 18, CH-2000 Neuchâtel, Switzerland |
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Abstract: | An analog of the peptidyl transferase inhibitor sparsomycin was a competitive inhibitor (Ki = 1.8 microM) of peptidyl-puromycin synthesis on E. coli polysomes. Preincubation of polysomes with the compound enhanced the degree of inhibition of peptide bond formation. A model for the involvement of a histidine residue in peptidyl transferase activity is presented as a result of our observations which include direct association of 3H] labelled analog with 70S ribosomes. The correct oxidation state of sulfur in the compound was necessary for the "preincubation effect" and entry of the compound into bacterial cells. |
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Keywords: | To whom all inquiries should be addressed |
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