Enrichment as a screening method for a high-growth-rate microalgal strain under continuous cultivation system |
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Authors: | Won-Sub Shin Hansol Lee Min-Gyu Sung Kwon-Tack Hwang Simon MoonGeun Jung Jong-Hee Kwon |
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Institution: | 1.Department of Chemical and Biomolecular Engineering,KAIST,Daejeon,Korea;2.Department of Food and Nutrition,Nambu University,Gwangju,Korea;3.Korea Research Institute of Chemical Technology,Daejeon,Korea;4.Department of Food Science & Technology and Institute of Agriculture & Life Science,Gyeongsang National University,Jinju,Korea |
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Abstract: | Microalgae are a promising feedstock for renewable biodiesel production. High productivity of biodiesel production from microalgae is directly related to growth rate as well as lipid content of cells. In the present study, an enrichment process in a continuous cultivation system was developed to screen a high-growth-rate microalga from a mixed culture of microalgal species; Chlorella vulgaris, Chlorella protothecoides, and Chlamydomonas reinhardtii were used as test organisms for our experiments. The time-dependent washout of mixed microalgal pool was executed to successfully enrich the C. reinhardtii, which exhibits the higher growth rate than C. vulgaris and C. protothecoides under turbidostat conditions within 75 h. The domination of C. reinhardtii in the mixed culture was validated by on-line monitoring of growth rate and flowcytometric analysis. For the time-efficient production of microalgal biomass, this screening process has a high potential to segregate the fast-growing microalgal strains from the pool of various uncharacterized microalgal species and random mutants. |
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