Demonstration of the formation of renin and renin-binding protein complex using high-performance liquid chromatography

A high-performance liquid chromatograph equipped with the newly developed gel, TSK G3000SW, was used to study the interaction between renin and renin-binding protein (RBP). Previously, the interaction could only be demonstrated after overnight gel chromatography in the presence of a non-physiological sulfhydryl reagent. However, this new high-speed gel chromatography provided a clear separation of renin and renin—RBP complex within 40 min. It also demonstrated that the renin—RBP complex was formed at 37°C in the absence of sulfhydryl reagent. These results indicate that the binding protein may play an important role in blood pressure regulation.