| Abstract: | A differential microrecirculation reactor was developed for kinetic analysis of both soluble and immobilized enzymes. The reactor system was easliy fabricated with in the laboratory from readily available materials. The disposable, small reactors allowed for in situ weight determination of the enzyme beads. Routinely, only a 1 ml liquid volume of substrate was used for each kinetic assay. The reactor system was also used for determination of partition coefficients. Both uses of the reactor system required only 5–10 min for completion of a given determination. |
