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A New Method For Axenic Mass Cultivation of Paramecium Tetraurelia*
Authors:  RGEN THIELE,OTMAR HONER-SCHMID D   ,JÜ  RGEN WAHL,GERTRUD KLEEFELD,JOACHIM SCHULTZ
Affiliation:JÜRGEN THIELE,OTMAR HONER-SCHMID D̊,JÜRGEN WAHL,GERTRUD KLEEFELD,JOACHIM SCHULTZ
Abstract:SYNOPSIS A method is described for the axenic mass cultivation of Paramecium tetraurelia strains 51s and 299s. the ciliate is grown in an enriched axenic medium developed by Soldo, Godoy & van Wagtendonk. Under continuous shaking on a rotary shaker, cultures were grown in one-liter Erlenmeyer flasks with 330 ml medium yield cell densities of 32,000 cell/ml and 20,000 cells/ml for strains 299s and 51s respectively. Doubling time is considerably shorter under these conditions than in the conventional static cultures. A 20-liter airlift bioreactor is described in detail which can be used successfully to otain up to 100 g wet weight of Paramecium in a single run; in this reactor the cell density reaches 38,000 cells/ml for strain 299s. and 23,000 cells/ml for 51s. This technic should facilitate the study of minor protein components of the ciliate.
Keywords:Paramecium tetraurelia strains 51s and 299s  axenic mass cultivation  bioreactor.
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