Oxidant Production by Human B Lymphocytes: Detection of Activity and Identification of Components Involved |
| |
| Authors: | Owen T G Jones Jon D Wood |
| |
| Institution: | aDepartment of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom;bInstitute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff, CF4 4XN, United Kingdom |
| |
| Abstract: | The O−2-generating NADPH oxidase, originally thought to be expressed only in phagocytic cells of the immune system, can also be expressed in B lymphocytes. Epstein–Barr virus-transformed B cell lines (EBV-BL) can generate O−2at rates corresponding to between 1 and 5% of the rates obtained by activated neutrophils. The composition of the NADPH oxidase of EBV-BL appears to be identical to that of phagocytic cells. In this report, methods are described for the establishment of EBV-BL, together with assays for the measurement of reactive oxidant production and detection of the constituent components of the NADPH oxidase in these cells. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
