不同年龄昆明白小鼠卵母细胞的生发泡互换

为探讨卵母细胞减数分裂异常及其与年龄相关变化之间的关系,对不同年龄段昆明白小鼠卵母细胞进行了生发泡(GV)移植研究。应用显微操作和电融合技术,将6~8周龄小鼠GV期卵母细胞分别与6月龄9、月龄和12月龄小鼠GV期卵母细胞进行GV互换,所形成的6种GV-胞质体复合体的融合率(89.7%~95.6%)和6种重组卵母细胞的成熟率(83.5%~88.2%)并不因小鼠年龄的改变而有所变化。成熟的6种重组卵母细胞经体外受精后,形成原核期胚和2-细胞期胚的比率(分别为80.0%~87.3%和42.7%~50.9%)并不因不同年龄小鼠卵母细胞GV互换所带来的细胞质或细胞核的改变而受到影响。

Germinal Vesicle Exchange among Oocytes from KM Mice at Different Ages

To investigate meiotic abnormity of oocytes and relationship between the abnormity and maternal age,germinal vesicle(GV) transfer was carried between GV oocytes from KM mice of different ages.Using micromanipulation and electrofusion procedures,GVs were exchanged between 6-8-week-old mice and 6-month-old mice,9-month-old mice,12-month-old mice,respectively.Electrofusion rates(89.7%-95.6%)were equivalent among 6 types of GV-cytoplast complexes constructed by GV exchange,and maturation rates(83.5%-88.2%)were not different among 6 types of the reconstructed GV oocytes.After in vitro maturation and fertilization of 6 types of the reconstructed oocytes,percentages of pronuclear-stage(80.0%-87.3%) and 2-cell embryos(42.7%-50.9%) were not different,suggesting that early embryo development is not influenced by cytoplasmic or nuclear changes among oocytes from mice of different ages.