Production of xanthan gum by Sphingomonas bacteria carrying genes from Xanthomonas campestris |
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Authors: | T J Pollock M Mikolajczak M Yamazaki L Thorne R W Armentrout |
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Institution: | (1) Shin-Etsu Bio, Inc, 6650 Lusk Blvd, Suite B106, San Diego, CA 92121, USA, US |
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Abstract: | Twelve genes coding for assembly, acetylation, pyruvylation, polymerization, and secretion of the polysaccharide xanthan
gum are clustered together on the chromosome of the bacterium Xanthomonas campestris. These genes (gumBCDEFGHIJKLM) are sufficient for synthesis of xanthan gum when placed in bacteria from a different genus, Sphingomonas. The polysaccharide from the recombinant microorganism is largely indistinguishable, structurally and functionally, from
native xanthan gum. These results demonstrate that a complex pathway for biosynthesis of a specific polysaccharide can be
acquired by a single inter-generic transfer of genes between bacteria. This suggests the biological and commercial feasibility
of synthesizing xanthan gum or other polysaccharides in non-native hosts.
Received 23 October 1996/ Accepted in revised form 14 April 1997 |
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Keywords: | : xanthan gum Xanthomonas campestris Sphingomonas exopolysaccharide complementation foreign gene expression |
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