| 新生隐球菌Cap59缺陷株ura5突变株的筛选方法 |
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| 引用本文： | 郭秀军,廖万清,任大明,王荫榆.新生隐球菌Cap59缺陷株ura5突变株的筛选方法[J].微生物学通报,2004,31(2):105-107. |
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| 作者姓名： | 郭秀军 廖万清 任大明 王荫榆 |
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| 作者单位： | 北京解放军306医院皮肤科,北京,100101 |
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| 基金项目： | 国家自然科学基金资助项目 (No 395 70 0 4 3) |
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| 摘 要： | 改进筛选新生隐球菌Cap59荚膜缺陷株ura5突变株的方法。采用硫酸二乙酯化学诱导新生隐球菌Cap59荚膜缺陷株，利用5-氟乳清酸(5-FOA)反筛选法筛选ura5尿嘧啶合成基因突变株。用新方法筛选到2株Cap59荚膜缺陷株ura5突变株。建立了一种筛选新生隐球菌荚膜缺陷株ura5突变株的简易方法。
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| 关 键 词： | 新生隐球菌 ura5突变株 |
| 文章编号： | 0253-2654(2004)02-0105-03 |
| 修稿时间： | 2003年5月30日 |
SELECTION FOR URA5 MUTANTS OF CRYPTOCCOCUS NEOFORMANS CAPSULE-DEFICIENT STRAIN CAP59 |
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| GUO Xiu-Jun,LIAO Wang-Qing,REN Da-Ming,WANG Yin-Yu.SELECTION FOR URA5 MUTANTS OF CRYPTOCCOCUS NEOFORMANS CAPSULE-DEFICIENT STRAIN CAP59[J].Microbiology,2004,31(2):105-107. |
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| Authors: | GUO Xiu-Jun LIAO Wang-Qing REN Da-Ming WANG Yin-Yu |
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| Abstract: | This study was to improve the way for selecting ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strains.They were induced by Diethyl Sulfate. Ura5 mutants were screened by 5-fluoroorotic acid counter selection method. Using the new method, we obtained two ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strain.A easy method that was used to screen ura5 mutants of Cryptoccocus neoformans has been established. |
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| Keywords: | Cryptoccocus neoformans ura5 mutant |
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