| 尼日利亚菌素高产菌株的选育 |
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| 引用本文： | 肖琳, 黄为一,. 尼日利亚菌素高产菌株的选育[J]. 微生物学通报, 2002, 29(4): 59-62 |
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| 作者姓名： | 肖琳 黄为一 |
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| 作者单位： | 南京农业大学资源与环境学院,南京,210095 |
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| 摘 要： | 通过对致死率和正负突变率的测定,确定了紫外线、亚硝酸、紫外线加吖啶橙3种诱变方式对吸水链霉菌NND-52的29号菌株进行诱变处理的合适剂量,并采用复合诱变得到了一株脱葡萄糖阻遏的高产菌株A19,其尼日利亚菌素的摇瓶产量比出发菌株提高了128％。传代结果表明,在传代的同时结合自然分离,可以保持稳定的产抗特性。
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| 关 键 词： | 尼日利亚菌素 复合诱变 脱葡萄糖阻遏 |
| 文章编号： | 0253-2654(2002)04-0059-05 |
| 修稿时间： | 2001-04-26 |
IMPROVEMENT OF NIGERICIN PRODUCTION IN STREPTOMYCES HYGROSCOPICUS NND-52 BY COMBINATION MUTANT |
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| XIAO Lin HUANG Wei-Yi. IMPROVEMENT OF NIGERICIN PRODUCTION IN STREPTOMYCES HYGROSCOPICUS NND-52 BY COMBINATION MUTANT[J]. Microbiology China, 2002, 29(4): 59-62 |
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| Authors: | XIAO Lin HUANG Wei-Yi |
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| Abstract: | Based on the lethal rate, positive and negative mutation rate, the optimal doses of UV, HNO2, UV + AO treating S. hygroscopicus NND-52-29 were determined. The mutant strain A19 that was obtained showed the derepres-sive property to glucose and the nigericin yield of A19 could be increased by 128%. When it was subcultured combined with natural screening, its stability was rather satisfied. |
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| Keywords: | Nigericin Combination mutant Glucose derepressive |
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