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Identification and isolation of vitamin K-dependent proteins by HPLC
Authors:R D Litwiller  R J Jenny  K G Mann
Institution:1. Section of Hematology Research, Mayo Clinic/Foundation, Rochester, Minnesota 55905 USA;2. Department of Biochemistry, University of Vermont, Burlington, Vermont 05405 USA;1. College of Resources and Environment, Chengdu University of Information Technology, Chengdu, 610225, China;2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China;3. State Environmental Protection Key laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China;1. Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK;2. Department of Biochemistry, University of Oxford, New Biochemistry Building, South Parks Road, Oxford, OX1 3QU, UK;1. Department of Chemistry, The University of Chicago, Chicago, Illinois, USA;2. Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA;3. Pritzker School of Medicine, The University of Chicago, Chicago, Illinois, USA;4. Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA
Abstract:Six of the seven known vitamin K-dependent proteins found in plasma were chromatographed on a large-pore propylsilane column using aqueous trifluoroacetic acid/acetonitrile gradients. Prothrombin and Factor VII coeluted, the others were readily resolved. The technique has been used to monitor the purification of protein C and protein S using immobilized anti-protein S. Preliminary evidence is presented which is suggestive of the existence of additional vitamin K-dependent proteins in plasma.
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