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Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
Authors:Paola L. Carvajal Monroy  Zipora Yablonka-Reuveni  Sander Grefte  Anne Marie Kuijpers-Jagtman  Frank A.D.T.G. Wagener  Johannes W. Von den Hoff
Affiliation:1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center;2Department of Biological Structure, University of Washington School of Medicine;3Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Abstract:Fibrosis and defective muscle regeneration can hamper the functional recovery of the soft palate muscles after cleft palate repair. This causes persistent problems in speech, swallowing, and sucking. In vitro culture systems that allow the study of satellite cells (myogenic stem cells) from head muscles are crucial to develop new therapies based on tissue engineering to promote muscle regeneration after surgery. These systems will offer new perspectives for the treatment of cleft palate patients. A protocol for the isolation, culture and differentiation of satellite cells from head muscles is presented. The isolation is based on enzymatic digestion and trituration to release the satellite cells. In addition, this protocol comprises an innovative method using extracellular matrix gel coatings of millimeter size, which requires only low numbers of satellite cells for differentiation assays.
Keywords:Developmental Biology   Issue 101   Head muscles   levator veli palatini muscle   digastric muscle   masseter muscle   satellite cells   isolation primary cells   cleft palate   regenerative medicine   tissue engineering   stem cells   differentiation   myofibers
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