Degradation of diphenylether by Pseudomonas cepacia |
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Authors: | Frank Pfeifer Sigrid Schacht Jürgen Klein Hans G Trüper |
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Institution: | (1) Bergbau-Forschung GmbH, Franz-Fischer-Weg 61, D-4300 Essen 13, Germany;(2) Institut für Mikrobiologie der Rheinischen Friedrich-Wilhelms-Universität, Meckenheimer Allee 168, D-5300 Bonn 1, Germany |
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Abstract: | The microbial degradation of hard coal implies the cleavage of diaryl ether linkages in the coal macromolecule. We investigated the biodegradation of diphenylether as a model compound representing this substructure of coal. A bacterial strain isolated from soil and identified as Pseudomonas cepacia, was able to grow with diphenylether as sole source of carbon. During microbial growth, three metabolites were detected in the culture supernatant by high pressure liquid chromatography. As product of ring hydroxylation and subsequent rearomatization, 2,3-dihydroxydiphenylether was identified by UV, mass and nuclear magnetic resonance spectrometry and gas chromatography analyses. The cleavage of the ether linkage led to the formation of phenol and 2-pyrone-6-carboxylic acid, the latter being not further degraded by Pseudomonas cepacia. The possible cleavage mechanism of the ether linkage is discussed.Non-standard abbreviations DPE
diphenylether
- PCA
2-pyrone-6-carboxylic acid
- GC
gas chromatography
- MS
mass spectrometry
- HPLC
high pressure liquid chromatography |
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Keywords: | Diphenylether Degradation pathway Phenol 2-Pyrone-6-carboxylic acid Ether cleavage Pseudomonas cepacia |
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