Electric fields affect the orientation of cortical microtubules and cell expansion in pea callus |
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Authors: | L M Blackman R L Overall |
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Institution: | (1) School of Biological Sciences, University of Sydney, 2006 Sydney, NSW, Australia |
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Abstract: | Summary Cortical microtubules in callus derived fromPisum sativum roots form parallel arrays within cells but are randomly oriented across the tissue. These arrays align perpendicular to the direction of an applied electric field of 6 mV per cell. Application of a field of 6 mV per cell for 4 days resulted in the co-ordinated expansion of cells parallel to the field direction. Cortical microtubule arrays were still aligned perpendicular to the applied field 24 h after removal of the field. The imposition of a field to callus after the removal of cortical microtubules by oryzalin and in the presence of the herbicide resulted in the orientation of recovering microtubules perpendicular to the direction of the field, indicating that microtubules are not directly involved in the detection of the field.Abbreviations EGTA
ethylene glycol-bis (-aminoethyl ether) N,N,N-tetraacetic acid
- FITC
fluorescein isothiocyanate
- MSB
microtubule stabilising buffer
- PIPES
piperazine-N,N-bis(2-ethanesulphonic acid)
- oryzalin
3,5-dinitro-N4,N4 dipropylsulphanil-amide |
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Keywords: | Co-ordinated cell expansion Cortical microtubules Electric fields Oryzalin Pea callus |
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