Effect of Ischemia on Heart Submitochondrial Superoxide Production |
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Authors: | C Guarnieri C Muscari C Ventura I Mavelli G Rotilio |
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Abstract: | NADH-dependent formation of superoxide anions (O-2) by rabbit cardiac submitochondrial particles (SMP) was stimulated after exposure of the isolated heart to 90 min of ischemic perfusion. This effect was more evident in the rotenone-inhibited region of the respiratory electron chain in comparison to the antimycin-inhibited region. The kinetic study of the NADH-dependent reaction showed that at the level of the rotenone-inhibited region, ischemia reduced Km value for NADH, differently from the antimycin-inhibited region where the kinetic constants remain unchanged. No significant changes of the Vmax values were observed in both SMP-producing O-2 sites.
The ischemic perfusions also produced a reduction of mitochondrial function, particularly evident when glutamate as substrate was studied. |
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Keywords: | superoxide mitochondria ischemia heart |
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