A simple and inexpensive dot-blotter for immunoblotting |
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| Affiliation: | 1. Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, OR, USA;2. Knight Cardiovascular Institute, Oregon Health and Science University, Portland, OR, USA;3. Ionis Pharmaceuticals Inc, Carlsbad, CA, USA;4. Aronora Inc, Portland, OR, USA;5. Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN, USA;1. Department of Nutrition and Food Science, Kobe Women''s University Graduate School of Life Sciences, 2-1 Higashisuma-aoyama, Suma-ku, Kobe 654-8585, Japan;2. Division of Signal Functions, Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;1. Department of Human Nutrition, Foods, and Exercise, Virginia Tech, Blacksburg, VA, United States;2. Virginia Tech Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States;3. Virginia Tech Faculty of Health Sciences, Virginia Tech, Blacksburg, VA, United States;4. Virginia Tech Metabolism Core, Virginia Tech, Blacksburg, VA, United States;5. Latvian Institute of Organic Synthesis, Laboratory of Pharmaceutical Pharmacology, Riga, Latvia;6. Department of Nutrition, Gillings School of Global Public Health and School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States |
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| Abstract: | The concept of specific binding of an antibody to an antigen can be understood easily through immuno-blotting experiments. For dot blotting the protein samples on a membrane, dot-blotters or filtration manifolds are necessary. However, such pieces of apparatus are complex, fragile and expensive. We report the simple and inexpensive construction and use of a dot-blotter using a magnetic rubber sheet. |
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