A simple laboratory experiment for teaching enzyme immobilization with urease and its application in blood urea estimation |
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| Institution: | 1. Polymer and Nano Research Laboratory, Department of Chemistry, Visva-Bharati University, Santiniketan 731235, India;2. Department of Zoology, Visva-Bharati University, Santiniketan 731235, India |
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| Abstract: | An experiment is described in which students carry out urease purification, immobilization and its application in blood urea estimation. Urease from pigeonpea is partially purified using acetone fractionation and then immobilized on calcium alginate in the form of beads. The immobilized enzyme has a better shelf-life at 4°C than soluble enzyme. Various aspects of enzyme immobilization are discussed. Blood urea estimation is carried out with immobilized enzyme beads and the beads can be used repeatedly for this purpose making it an economical procedure compared to commercial kits. |
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