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Root membrane thermostability of Cornus florida L. provenances
Institution:1. University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Dositeja Obradovi?a 3, 21000 Novi Sad, Serbia;2. University of Belgrade, Institute of Botany and Botanical Garden “Jevremovac”, Takovska 43, 11000 Belgrade, Serbia;3. Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia;1. Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang 550025, China;2. Statistical Genomic Lab, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;3. Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070, China;4. Department of Entomology, University of Agriculture Faisalabad, Pakistan;5. King Khalid University, College of Science, Department of Biology, Abha 61413, Saudi Arabia;6. Assiut University, Faculty of Science, Botany and Microbiology Department, Assiut 71516, Egypt;7. Department of Plant Protection, Faculty of Agricultural Sciences, Ghazi University Dera Ghazi Khan Punjab, Pakistan
Abstract:Flowering dogwood seed collected from native trees in 3 USDA Hardiness Zones (6b, 7a, and 8a; American Horticultural Society (AHS) Heat-Zones 7, 7, and 8, respectively) were germinated and grown in containers prior to laboratory procedures to determine root cell membrane thermostability. Unsuberized, current season, fine root tissues were subjected to temperatures ranging from 20 to 60°C for 30 min and analyzed for cellular electrolyte leakage. Electrolyte leakage from root tissue exhibited a sigmoidal response to temperature for trees from each location. Critical midpoint temperature (Tm) was greater for seedlings native to USDA Hardiness Zone 6b (AHS Heat-Zone 7), 52.4±0.6°C, than Tm for seedlings originating from USDA Zone 7a (AHS Zone 7), 51.2±0.5°C. However, seedlings from USDA Zone 8a (AHS Zone 8), at 51.5±0.4°C, were similar to those collected in USDA Zones 6b (AHS Zone7) and 7a (AHS Zone 7). The results of this study find little genetic variability across this part of the native range of flowering dogwood regarding root thermotolerance. Although differences between two provenances were found, these differences may be of little ecological significance.
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