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Sex determination using the polymerase chain reaction
Institution:1. Departamento de Química Inorgánica, Facultad de Ciencias, Universidad de Granada, Avda. Fuentenueva, 18071 Granada, Spain;2. Departamento de Biología y Geología, Física y Química Inorgánica, Universidad Rey Juan Carlos, C/Tulipán s/n, 28933 Móstoles, Madrid, Spain;3. Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Avda. Fuentenueva, 18071 Granada, Spain;4. Departamento de Química Aplicada, Facultad de Química, Universidad del País Vasco UPV/EHU, 20018 Donostia-San Sebastián, Spain;5. Departamento de Química Analítica, Facultad de Ciencias, Universidad de Granada, Avda. Fuentenueva, 18071 Granada, Spain;6. Laboratorio de Estudios Cristalográficos, IACT, CSIC-Universidad de Granada, Avda. de las Palmeras 4, 18100 Armilla, Granada, Spain;7. Department of Chemistry and Physics, Research Centre CIAIMBITAL, University of Almería, Ctra. Sacramento s/n, 04120 Almería, Spain;1. Child Health Research Foundation, Department of Microbiology, Dhaka Shishu Hospital, Dhaka 1207, Bangladesh;2. International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA;3. Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA;4. Respiratory Diseases Branch, US Centers for Disease Control and Prevention, Atlanta, GA, USA;5. Bangladesh Institute of Child Health, Dhaka Shishu Hospital, Dhaka, Bangladesh;3. Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095;4. Molecular Biology Institute, UCLA, Los Angeles, California 90095;5. Department of Energy Institute for Genomics and Proteomics, UCLA, Los Angeles, California 90095;6. Department of Chemistry and Biochemistry, California State University, Fullerton, California 92831;1. Dipartimento di Medicina Interna e Specialità Mediche, Sapienza University of Rome, Italy;2. Istituto Pasteur Italia – Fondazione Cenci Bolognetti, Rome, Italy;3. Center for Life Nano Science, Istituto Italiano di Tecnologia, Rome, Italy;1. Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany;2. German Centre for Infection Research, Hannover, Germany (DZIF), Partner-site Hannover-Braunschweig, Germany;3. German Centre for Infection Research (DZIF), External Partner Site Frankfurt, Germany;4. Centre for Individualised Infection Medicine (CIIM), Hannover, Germany;5. Department of Internal Medicine 1, University Hospital Frankfurt, Frankfurt, Germany;6. Department of Gastroenterology and Hepatology, Essen University Hospital, Essen, Germany;7. Integrated Research and Treatment Centre Transplantation (IFB-Tx), Hannover Medical School, Hannover, Germany
Abstract:A practical class experiment on the PCR is described which has been used over several years as part of an undergraduate biochemistry and molecular biology course for science students. A major aim is to provide experience in the use of the polymerase chain reaction (PCR) and its interpretation. Students are given small coded DNA samples and use the PCR reaction to determine whether the sample is from a male or a female.
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