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Study of the metabolism of pyrazinamide using a high-performance liquid chromatographic analysis of urine samples |
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| Authors: | Tetsuya Yamamoto Yuji Moriwaki Sumio Takahashi Toshikazu Hada Kazuya Higashino |
| Institution: | 1. Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62271, México;2. Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China;3. Center for Arrhythmia Research, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA;4. CONACYT- Laboratorio de Ecología Molecular Microbiana, Centro de Investigaciones en Ciencias Microbiológicas-Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, C.P. 72570 Puebla, México |
| Abstract: | A reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of pyrazinamide and its metabolites in urine. Study of the metabolism of pyrazinamide by this method demonstrated that 5-hydroxypyrazinamide excretion was compatible with pyrazinoic acid excretion and allopurinol decreased in vivo conversion of pyrazinamide to 5-hydroxypyrazinamide and blocked that of pyrazinoic acid to 5-hydroxypyrazinoic acid. |
| Keywords: | pyrazinamide pyrazinoic acid 5-hydroxypyrazinamide 5-hydroxypyrazinoic acid high-performance liquid chromatography xanthine oxidase xanthinuria allopurinol |
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