Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp. |
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Authors: | J Loureiro D Kopecký S Castro C Santos P Silveira |
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Institution: | (1) CESAM & Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;(2) Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic |
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Abstract: | Festuca L. has an important diversification centre in the Iberian Peninsula. We used chromosome counting, fluorescence (FISH) and
genomic in situ hybridization (GISH), and DNA flow cytometry (FCM) to clarify the taxonomic position of several taxa, to search
for phylogenetic relationships and to assess the extent and pattern of genome variation in fescues. The chromosome number
of Festuca duriotagana var. barbata is determined for the first time and new ploidy level estimations are given for F. rothmaleri and F. summilusitana. In the latter species, besides the reported decaploid level, dodecaploidy was found in some populations, which points to
the existence of an unrecognized taxon. Moreover, these differences were confirmed by FCM and a high positive correlation
was found with the type of substrate where F. summilusitana was growing. For each section, a decrease of genome size with increase of polyploidy was observed. In general, in situ hybridization
techniques failed to reveal phylogenetic relationships among the selected species. In FISH, a variation in the number of rDNA
sites was observed in some species. GISH results indicate that F. henriquesii is not a progenitor of the studied polyploid species. |
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Keywords: | Fescues Festuca flow cytometry genome size Iberian Peninsula karyology in situ hybridization molecular cytogenetics |
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