Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system |
| |
Authors: | Hee-Jong Woo Hyun-Suk Cho Sun-Hyung Lim Kong-Sik Shin Si-Myung Lee Ki-Jong Lee Dong-Hern Kim Yong-Gu Cho |
| |
Institution: | (1) Biosafety Division, National Academy of Agricultural Science, Rural Development Administration, 150 Sooin-ro, Seodun-dong, Gwonseon-gu, Suwon, 441-707, South Korea;(2) Department of Crop Science, Chungbuk National University, Cheongju, 361-763, South Korea |
| |
Abstract: | Antibiotic resistance marker genes are powerful selection tools for use in plant transformation processes. However, once transformation
is accomplished, the presence of these resistance genes is no longer necessary and can even be undesirable. We herein describe
the successful excision of antibiotic resistance genes from transgenic plants via the use of an oxidative stress-inducible
FLP gene. FLP encodes a recombinase that can eliminate FLP and hpt selection genes flanked by two FRT sites. During a transformation procedure in tobacco, transformants were obtained by selection on hygromycin media. Regenerants
of the initial transformants were screened for selective marker excision in hydrogen peroxide supplemented media and both
the FLP and hpt genes were found to have been eliminated. About 13–41% of regenerated shoots on hydrogen peroxide media were marker-free.
This auto-excision system, mediated by the oxidative stress-inducible FLP/FRT system to eliminate a selectable marker gene can be very readily adopted and used to efficiently generate marker-free transgenic
plants. |
| |
Keywords: | Auto-excision FLP/FRT recombination system Marker-free Oxidative stress-inducible Transgenic tobacco |
本文献已被 SpringerLink 等数据库收录! |
|