Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery |
| |
| Authors: | Lee Hyoung-Joo Kang Min-Jeong Lee Eun-Young Cho Sang Yun Kim Hoguen Paik Young-Ki |
| |
| Affiliation: | Department of Biochemistry, Yonsei Proteome Research Center and Biomedical Proteome Research Center, Yonsei University, Seoul, Korea. |
| |
| Abstract: | A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
