| HMBA诱导人肝癌SMMC—7721细胞分化的观察 |
| |
| 引用本文： | 欧阳高亮,李祺福,等.HMBA诱导人肝癌SMMC—7721细胞分化的观察[J].实验生物学报,2001,34(4):269-273. |
| |
| 作者姓名： | 欧阳高亮 李祺福 |
| |
| 作者单位： | [1]厦门大学生命科学学院细胞生物学教研室，厦门361005 [2]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室，厦 |
| |
| 摘 要： |
|
| 关 键 词： | HMBA 肝癌细胞 诱导分化 SMMC－7721细胞 |
Differentiation of human hepatocarcinoma SMMC-7721 cells induced by HMBA] |
| |
| G L Ouyang,Q F Li,X X Peng,S G Hong.Differentiation of human hepatocarcinoma SMMC-7721 cells induced by HMBA][J].Acta Biologiae Experimentalis Sinica,2001,34(4):269-273. |
| |
| Authors: | G L Ouyang Q F Li X X Peng S G Hong |
| |
| Institution: | Laboratory of Cell Biology, Key Laboratory of Ministry of Education for Cell Biology and Tumor Engineering, School of Life Sciences, Xiamen University, Xiamen 361005. |
| |
| Abstract: | In this paper, the effects of HMBA on the differentiation of human hepatocarcinoma cell line SMMC-7721 were investigated. After treated with 5 mmol/L HMBA, the proliferation of SMMC-7721 cells was inhibited remarkably, the cell growth inhibitory rate amounted to 64.14%, the cell mitotic index was declined by 53.88%. Light microscopy and transmission electron microscopy showed that the morphology and ultrastructure of the cells treated with HMBA undergone restorational alteration. Cytochemistry and immunocytochemistry assay revealed that the activities of gamma-GT declined and the levels of AFP and PCNA downregulated while the activity of TAT increased significantly after HMBA treatment. In the meantime, flow cytometry analysis showed that HMBA could arrest the cells in G0/G1 phase. The results showed that HMBA could effectively inhibit the proliferation, reverse the malignant morphology and ultrastructure, alter the levels of enzymes and antigens, arrest the cells in G0/G1, and induce the differentiation of human hepatocarcinoma SMMC-7721 cells in vitro. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
|
