Triple fixation of Bacillus subtilis dormant spores. |
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Authors: | S Kozuka and K Tochikubo |
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Abstract: | A triple-fixation method with a sequential application of 5% glutaraldehyde, 1% osmium tetroxide, and 2% potassium permanganate gave superior preservation of the ultrastructure of Bacillus subtilis dormant spores with a thick spore coat. |
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