Hepatic and extra-hepatic glutathione-S-transferase activity in wild pigeons (Columba livia) |
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Authors: | Ashwini Kumar M. M. Husain Hasan Mukhtar C. R. Krishna Murti |
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Affiliation: | (1) Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Post Box No. 80, 226 001 Lucknow |
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Abstract: | Glutathione-S-transferase (EC 2.5.1.18) activity was assayed in hepatic and extra-hepatic tissues of pigeons using l-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Gluthathione-S-transferase activity towards 1-chloro-2,4-dinitrobenzene in pigeon was in the order: kidney > liver > testes > brain > lung> heart. The enzyme activity with 1-chloro-2,4-dinitrobenzene as substrate was 40–44 times higher in pigeon liver and kidney than that observed with 1,2-dichloro-4-dinitrobenzene as substrate.K m values of hepatic and renal glutathione transferase with l-chloro-2,4-dinitrobenzene as substrate were 2.5 and 3 mM respectively. Double reciprocal plots with varying reduced gluthathione concentrations resulted in biphasic curves with twoK m values (liver 0.31 mM and 4mM; kidney 0.36 mM and 1.3 mM). The enzyme activity was inhibited by oxidized gluthathione in a dose-dependent pattern. 3-Methylcholanthrene elicited about 50% induction of hepatic glutathione transferase activity whereas phénobarbital was ineffective. |
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Keywords: | Avian glutathione-S-transferase oxidized glutathione 3-methylcholanthrene phenobarbital inhibition by oxidized glutathione glutathione-S-transferase |
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