Interference by DDT and cyclodiene types of insecticides with chloroplast-associated reactions |
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Authors: | D E Moreland W P Novitzky |
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Institution: | 1. Centro di Studio per l''Istochimica del C.N.R., Dipartimento di Biologia Animale, Università di Pavia Italy;2. Centro di Studio per l''Elettronica Quantistica e la Strumentazione Elettronica del C.N.R., Istituto di Fisica, Politecnico di Milano Italy |
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Abstract: | The effects of DDT, some of its analogs, and selected cyclodiene insecticides on isolated spinach (Spinacea oleracea L.) thylakoids were identified, characterized, and compared to responses induced by selected herbicides. Except for endrin, the insecticides inhibited light-induced electron transport, altered chlorophyll fluorescence transients, and competitively displaced 14C]atrazine 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine], a known photosystem II inhibitor, from the membranes. The insecticides appeared to act at, or near B, the secondary electron acceptor of photo-system II. Binding of DDT and dieldrin was estimated at 900 and 2200 molecules, respectively, per photosynthetic unit (490 chlorophyll molecules). The insecticides also inhibited valinomycin-induced swelling of the thylakoid membrane. Whereas inhibition of electron transport can be attributed to interaction by the insecticides with a proteinaceous component of the thylakoid membrane, interference with the action of valinomycin may involve interaction with lipoidal constituents of the membrane. |
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Keywords: | Hematoporphyrin-derivative Porphyrin species Culture medium Spectroscopic studies Time-resolved fluorimetry HpD hematoporphyrin-derivative NPS new porphyrin species PHA phytohemagglutinin |
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