A liposome-PCR assay for the ultrasensitive detection of biological toxins |
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Authors: | Mason Jeffrey T Xu Lixin Sheng Zong-mei O'Leary Timothy J |
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Affiliation: | Department of Biophysics, Armed Forces Institute of Pathology, 1413 Research Boulevard, Rockville, Maryland 20850, USA. mason@afip.osd.mil |
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Abstract: | We describe an ultrasensitive immunoassay for detecting biotoxins that uses liposomes with encapsulated DNA reporters, and ganglioside receptors embedded in the bilayer, as a detection reagent. After immobilization of the target biotoxin by a capture antibody and co-binding of the detection reagent, the liposomes are ruptured to release the reporters, which are quantified by real-time PCR. Assays for cholera and botulinum toxins are several orders of magnitude more sensitive than current detection methods. |
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