| 枯草芽孢杆菌发酵条件优化及其破乳效能 |
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| 引用本文： | 代阳,魏利,王继华,马放,徐暘.枯草芽孢杆菌发酵条件优化及其破乳效能[J].微生物学通报,2010,37(4):0580-0585. |
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| 作者姓名： | 代阳 魏利 王继华 马放 徐暘 |
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| 作者单位： | 1. 哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨,150090
2. 哈尔滨师范大学,生命科学与技术学院,黑龙江哈尔滨150025 |
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| 基金项目： | 国家863计划项目(No. 2009AA062906); 黑龙江省普通高等学校青年学术骨干支持计划项目(No. 1154G31); 博士后研究人员落户黑龙江科研启动金资助项目(2009) |
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| 摘 要： | 本文对枯草芽孢杆菌在不同碳源、氮源培养基的生长及破乳效能进行了研究, 并通过正交试验对枯草芽孢杆菌的发酵条件进行优化结果表明, 单一碳源葡萄糖和混合碳源葡萄糖 + 液体石蜡的培养基可提高枯草芽孢杆菌的发酵产量; 单一碳源葡萄糖、混合碳源葡萄糖 + 汽油和以硝酸铵 + 酵母膏为氮源的菌液有较高的破乳效能; 在正交试验中, 培养温度对枯草芽孢杆菌的发酵产量影响最大, 其最优组合为: 培养温度25oC, 摇床转数140 r/min, 培养pH值7.0, 接菌量6 mL, 培养时间24 h。摇床转数对枯草芽孢杆菌的发酵产物的破乳效能影响最大, 优化结果为: 培养温度25oC, 摇床转数140 r/min, pH值7.0, 培养时间20 h。
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| 关 键 词： | 枯草芽孢杆菌 生物破乳剂 发酵条件 破乳效能 |
Optimization of Fermentation Conditions for Bacillus subtilis and Its Demulsification Efficiency |
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| DAI Yang,WEI Li,WANG Ji-Hu,MA Fang and XU Yang.Optimization of Fermentation Conditions for Bacillus subtilis and Its Demulsification Efficiency[J].Microbiology,2010,37(4):0580-0585. |
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| Authors: | DAI Yang WEI Li WANG Ji-Hu MA Fang and XU Yang |
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| Institution: | 1. Harbin Institute of Technology Urban Water Resources and State Key Laboratory of Water Environment, Harbin, Heilongjiang 150090, China;1. Harbin Institute of Technology Urban Water Resources and State Key Laboratory of Water Environment, Harbin, Heilongjiang 150090, China;2. Life Science and Technology College, Harbin Normal University, Harbin, Heilongjiang 150025, China;1. Harbin Institute of Technology Urban Water Resources and State Key Laboratory of Water Environment, Harbin, Heilongjiang 150090, China;1. Harbin Institute of Technology Urban Water Resources and State Key Laboratory of Water Environment, Harbin, Heilongjiang 150090, China |
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| Abstract: | Growth and de-emulsification efficiency of Bacillus subtilis under different carbon and nitrogen sources were investigated. The fermentation conditions were also optimized with orthogonal tests. The results showed that medium containing glucose as sole carbon source and compound medium containing glucose and liquid paraffin as carbon sources could enhance the fermentation production of the Bacillus subtilis. Higher de-emulsification efficiency was obtain with medium utilized glucose as sole carbon source, c... |
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| Keywords: | Bacillus subtilis Biological de-emulsifier Fermentation conditions De-emulsification activity |
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