Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus |
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| Authors: | N Okazaki Y Hibino Y Asano M Ohmori N Numao K Kondo |
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| Affiliation: | Sagami Chemical Research Center, Kanagawa, Japan. |
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| Abstract: | The gene coding for phenylalanine dehydrogenase [PDH; L-phenylalanine: NAD+ oxidoreductase (deaminating); EC 1.4.1.-] from Bacillus sphaericus SCRC-79a was cloned onto plasmid pUC9, and the nucleotide sequence of the 2-kb DNA region of the insert was determined. A 1143-bp open reading frame consisting of 381 codons was identified as a pdh gene coding for PDH. |
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