Strategy for Stabilizing Enzymes Part One: Increasing Stability of Enzymes via their Multi-Point Interaction with a Support |
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Authors: | V. V. Mozhaev N. S. Melik-nubarov M. V. Sergeeva Virginius ik nis Karel Martinek |
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Affiliation: | a Chemistry Department, Moscow State University, Moscow, USSRb Institute of Biochemistry, USSR Academy of Sciences, Moscow, USSRc All-Union Research Institute of Applied Enzymology, Vilnius, Lithuanian, SSRd Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague, Czechoslovakia |
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Abstract: | This review states that the covalent multi-point attachment of enzymes to a support is the most general approach to stabilize them against different denaturing conditions, namely against their inactivation caused by protein unfolding. It is suggested that the change in the wavelength of the maximum emission in fluorescence spectra of a protein, resulting from its denaturation, can be used to evaluate a priori the effectiveness of stabilization. The copolymerization method of enzyme immobilization, as the most promising approach to stabilizing enzymes, is discussed in detail. |
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Keywords: | Enzymes immobilization stabilization |
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