| 用RACE技术快速扩增五指山猪来源的猪内源性反转录病毒3＇LTR |
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| 引用本文： | 吕茂民,谢放,吴健敏,章金刚. 用RACE技术快速扩增五指山猪来源的猪内源性反转录病毒3＇LTR[J]. 生物技术通讯, 2004, 15(4): 354-356 |
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| 作者姓名： | 吕茂民 谢放 吴健敏 章金刚 |
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| 作者单位： | 军事医学科学院野战输血研究所 北京100850(吕茂民，谢放，吴健敏)，军事医学科学院野战输血研究所 北京100850(章金刚) |
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| 基金项目： | 国家自然科学基金(30270989)，北京市自然科学基金(5032015)，国家社会公益研究专项资金(2001DIA40036) |
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| 摘 要： | 测定我国小型猪来源的猪内源性反转录病毒(PERV)3'LTR,以便于PERV全基因的克隆和分析。用cDNA末端快速扩增(RACE)技术,从五指山猪外周血淋巴细胞mRNA中扩增到PERV-3'LTR,并克隆入pGEM-Teasy载体,将阳性克隆进行序列测定和同源性分析。测序结果显示该克隆3'端的尾部有一个由12个A组成的poly(A)信号;其R区与PERV-MSL的R区(约64bp)基本一致,同源性分析表明其与PERV-MSL的3'LTR具有81%的序列同源性。说明成功扩增了我国五指山猪来源的PERV-3'LTR,将有利于PERV全基因的克隆。
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| 关 键 词： | RACE 五指山猪 猪内源性反转录病毒 3’长末端重复 同源性分析 |
| 文章编号： | 1009-0002(2004)04-0354-03 |
| 修稿时间： | 2003-08-20 |
Sequence determination and analysis of 3'LTR of porcine endogenous retrovirus using rapid amplification of cDNA ends |
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| LV Mao-min,XIE Fang,WU Jian-min,ZHANG Jin-gang Institute of Transfusion Medicine,Academy of Military Medical Sciences,Beijing,China. Sequence determination and analysis of 3'LTR of porcine endogenous retrovirus using rapid amplification of cDNA ends[J]. Letters in Biotechnology, 2004, 15(4): 354-356 |
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| Authors: | LV Mao-min XIE Fang WU Jian-min ZHANG Jin-gang Institute of Transfusion Medicine Academy of Military Medical Sciences Beijing China |
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| Affiliation: | LV Mao-min,XIE Fang,WU Jian-min,ZHANG Jin-gang Institute of Transfusion Medicine,Academy of Military Medical Sciences,Beijing100850,China |
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| Abstract: | In order to analyzed the full-length genomic sequence of porcine endogenous retrovirus.Total RNA were extract-ed from peripheral blood lymphocyte of wu-zhi-shan pig by TRIzol Reagent,and isolated the mRNA from total RNA.Us-ing the RACE method,the PERV-3'LTR of wu-zhi-shan pig of China we re amplified and cloned.Sequence analysis shown that the3'LTR have81%sequence homology with PERV-MSL.The results showed that the PERV-3'LTR from wu-zhi-shan pig of China have been cloned successfully. |
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| Keywords: | rapid amplification of cDNA ends porcine endogenous retrovirus 3' long terminal repeat wu-zhi-shan pig |
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