Chemical force microscopy with active enzymes |
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Authors: | Fiorini M McKendry R Cooper M A Rayment T Abell C |
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Institution: | Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom. |
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Abstract: | The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy. |
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