GRP94 reduces cell death in SH-SY5Y cells perturbated calcium homeostasis |
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Authors: | Y. Bando T. Katayama A. N. Aleshin T. Manabe M. Tohyama |
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Affiliation: | Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan. ybando@asahikawa-med.ac.jp |
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Abstract: | The endoplasmic reticulum (ER) resident-94 kDa glucose-regulated protein (GRP94), plays a pivotal role in cell death due to ER stress. In our study expression of GRP94 was increased in human neuroblastoma SH-SY5Y cells due to exposure to calcium ionophore A23187. A23187-mediated cell death was associated with activation of the major cysteine proteases, caspase-3 and calpain. Pretreatment with adenovirus-mediated antisense GRP94 (AdGRP94AS) reduced viability of SH-SY5Y cells subjected to A23187 treatment compared with wild type cells or cells with adenovirus-mediated overexpression of GRP94 (AdGRP94S). These results indicated that suppression of GRP94 is associated with accelerated cell death. Moreover, expression of GRP94 suppressed A23187-induced cell death and stabilized calcium homeostasis. |
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Keywords: | calcium homeostasis endoplasmic reticulum molecular chaperone neuronal cell death |
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