Identification of a new quaternary association for legume lectins |
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Authors: | Moreno Frederico Bruno Mendes Batista de Oliveira Taianá Maia Martil Daiana Evelin Viçoti Magno Massolino Bezerra Gustavo Arruda Abrego José Ramon Beltran Cavada Benildo Sousa Filgueira de Azevedo Walter |
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Affiliation: | Curso de Pós-gradua??o em Biofísica Molecular, Departamento de Física, Universidade Estadual Paulista Júlio de Mesquita Filho, R. Cristov?o Colombo, 2265, Nazareth, S?o José do Rio Preto, S?o Paulo, Brazil. |
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Abstract: | Lotus tetragonolobus lectin (LTA) is a fucose-specific legume lectin. Although several studies report a diverse combination of biological activities for LTA, little is known about the mechanisms involved in l-fucosyl oligosaccharide recognition. The crystal structure of LTA at 2.0A resolution reveals a different legume lectin tetramer. Its structure consists of a homotetramer composed of two back-to-back GS4-like dimers arranged in a new mode, resulting in a novel tetramer. The LTA N-linked carbohydrate at Asn4 and the unusual LTA dimer-dimer interaction are related to its particular mode of tetramerization. In addition, we used small angle X-ray scattering to investigate the quaternary structure of LTA in solution and to compare it to the crystalline structure. Although the crystal structure of LTA has revealed a conserved metal-binding site, its l-fucose-binding site presents some punctual differences. Our investigation of the new tetramer of LTA and its fucose-binding site is essential for further studies related to cross-linking between LTA and complex divalent l-fucosyl carbohydrates. |
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