Evidence that the cytosolic activity of 3-hydroxybutyrate dehydrogenase in chicken liver isL-3-hydroxyacid dehydrogenase |
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Authors: | G.R. Herzberg M. Gad |
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Affiliation: | Department of Biochemistry, Memorial University of Newfoundland, St. John''s, Newfoundland, A1B 3X9 Canada |
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Abstract: | Classical fractionation studies showed that chicken liver contains two enzymes which can oxidize DL-3-hydroxybutyrate. The cytosolic enzyme is specific for the L-(+) isomer and accounts for 60% of the total activity. The mitochondrial activity is specific for the D-(?) isomer and accounts for 40% of the total activity. Kinetic studies showed that L-gulonic acid is a competitive inhibitor of the enzyme. We conclude that the cytosolic enzyme is the previously described L-3-hydroxyacid dehydrogenase. |
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Keywords: | Hydroxybutyrate dehydrogenase Hydroxyacid dehydrogenae (Chicken liver) Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid |
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