Structure of the Saccharomyces cerevisiae cell wall: Mannoproteins released by zymolyase and their contribution to wall architecture |
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Authors: | F.I. Javier Pastor Eulogio Valentín Enrique Herrero Rafael Sentandreu |
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Affiliation: | Departamento de Microbiología, Facultad de Farmacia, Universidad de Valencia, Avda Blasco Ibáñez 13, Valencia 46010 Spain |
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Abstract: | Purified zymolyase containing β-glucanase activity preferentially released a 29 kDa mannoprotein from isolated yeast cell walls and a high-molecular-mass (greater than 120 kDa) material. Endo-β-N-acetylglucosaminidase H digestion indicated that the 29 kDa mannoprotein contains a unique core coligosaccharide N-glycosidically linked to a 26 kDa peptide moiety. Cells grown in the presence of tunicamycin incorporated the nonglycosylated 26 kDa peptide into the wall, but not the large mannoprotein molecules. Treatment of isolated walls with SDS solubilized more than 30 different mannoproteins, one of tehm being the 29 kDa species, but the large-size molecules were not affected. Regenerating protoplasts incorporated into the forming walls most of the SDS-solubilizable species seen in mature cell walls, but the zymolyase-solubilizable mannoproteins were absent. Wall mannoproteins have also been compared with those of the periplasmic space, most of the species being commonly present at both compartments. Turnover of individual species has been studied by pulse and chase experiments. While mannoproteins from the walls remain stable for long periods, periplasmic molecules exhibit a rapid turnover rate. |
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Keywords: | Cell wall structure Mannoprotein Zymolyase (S. cerevisiae) PMSF phenylmethylsulfonyl fluoride SDS sodium dodecyl sulfate |
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