Dissociation of yeast 80 S ribosomes by chaotropic salts |
| |
Authors: | John E Kinsella |
| |
Institution: | Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853 U.S.A. |
| |
Abstract: | Exposure of yeast 80 S ribosomes to chaotropic salts such as NaClO4 or NaSCN at concentrations as low as 0.4 M resulted in complete dissociation and subsequent aggregation of the ribosomal proteins. However, under similar conditions, both NaCl and NaBr did not cause dissociation and aggregation. The protein precipitate obtained by exposing the ribosomes to 0.5 M NaClO4 was free of any rRNA contamination as judged by ultraviolet-absorption analysis. Comparison of the two-dimensional polyacrylamide gel electrophoretic analysis of the above ribosomal protein precipitate with that ribosomal proteins isolated by the standard acetic acid extraction procedure revealed that the protein precipitate contained all the ribosomal proteins. Based on these results, a simple method for the isolation of total ribosomal proteins and rRNA under mild, nondenaturing conditions is proposed. A possible mechanism for the dissociation of proteins from the ribosome by chaotropic salts is also discussed. |
| |
Keywords: | Chaotropic salt Ribosome dissociation (Yeast) |
本文献已被 ScienceDirect 等数据库收录! |
|