The effects of monocyte-conditioned medium and interleukin 1 on the synthesis of collagenous and non-collagenous proteins by mouse bone and human bone cells in vitro |
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Authors: | J.N. Beresford J.A. Gallagher M. Gowen M. Couch J. Poser D.D. Wood R.G.G. Russell |
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Affiliation: | 1. Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX U.K.;2. Procter & Gamble Co., Cincinnati, OH, USA;3. Merck, Sharp & Dohme Research Laboratory, PO Box 2000, Rahway, NJ 07065 U.S.A. |
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Abstract: | Cultural adherent human mononuclear cells produce factor(s) which stimulate the release of calcium from new-born mouse calvaria in organ culture. This stimulation of bone resorption is accompanied by an inhibition of the incorporation of [3H]proline into collagen which is independent of increased prostaglandin production by the bone. When human osteoblast-like cells are treated with conditioned medium from human mononuclear cells, collagen accounts for a decreased proportion of the protein synthesised. This effect on matrix synthesis is not accompanied by an inhibitory action of the monocyte-conditioned medium preparations on net cell proliferation. In human osteoblast-like cell cultures, partially purified human interleukin 1 also inhibits the production of the bone-specific protein osteocalcin in a dose-dependent fashion. These observations are consistent with the hypothesis that products of human monocytes similar to, or identical with, human interleukin 1 may be important regulators of bone metabolism and may contribute to the bone loss seen in diseases such as chronic rheumatoid arthritis. |
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Keywords: | Monocyte-conditioned medium Interleukin 1 Collagen Protein synthesis (Bone cell) BAPN β-aminoproprionitrile fumarate MCF ononuclear cell factor |
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