The effects of deuterium oxide (2H2O) on the polymerization of tubulin in vitro |
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Authors: | Tomohiko J. Itoh Hidemi Sato |
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Affiliation: | Sugashima Marine Biological Laboratory, Sugashima-cho, Toba-shi, Mie 517 Japan |
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Abstract: | The initial rate and final extent of polymerization of both bovine brain tubulin and sea urchin egg tubulin were enhanced in the presence of 2H2O. The yields were increased in association with the elevation of the 2H2O concentration. 2H2O also reduced the critical concentration for polymerization of brain tubulin. Thermodynamic analysis was attempted using the temperature dependence of the critical concentration for polymerization in the presence of 2H2O. We obtained linear van 't Hoff plots and calculated thermodynamic parameters which were positive and were increased with the elevation of the 2H2O concentration. The enhancement of the polymerization of tubulin by 2H2O could, therefore, be the result of the strenghening of intra-and/or inter-molecular hydrophobic interactions of the tubulin molecules. We believe that the increase in lenghth and number of microtubules of the mitotic spindles in the dividing cells of the eukaryotes with 2H2O may be caused by the direct involvement of 2H2O in the polymerization of tubulin. |
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Keywords: | Tubulin polymerization Deuterium oxide Mes, 4-morpholineethanesulfonic acid Pipes, 1,4-piperazinediethanesulfonic acid SDS, sodium dodecyl sulfate |
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