Immunological identification of soluble carbonyl reductase with testosterone 17β-dehydrogenase (NADP+) in guinea pig liver and kidney |
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| Authors: | Hideo Sawada Akira Hara Masakazu Hayashibara Toshihiro Nakayama Shigeyuki Usui |
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| Institution: | Department of Biochemistry, Gifu Pharmaceutical University, Mitahora-higashi, Gifu 502, Japan |
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| Abstract: | Antiserum was produced against one of two carbonyl reductases purified from guinea pig liver cytosol to identify the enzymes as testosterone 17β-dehydrogenase isozymes. Immunoelectrophoresis and immunoprecipitation with the antiserum indicated that the two reductases had common antigenic sites. The antiserum inhibited most of both carbonyl reductase and testosterone 17β-dehydrogenase activities in the purified reductases and in cytosols of liver and kidney. |
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| Keywords: | Carbonyl reductase Testosterone dehydrogenase (Guinea pig) |
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