金藻基因组DNA的提取与PCR扩增

以金藻门群体和单细胞藻类的典型代表--球形棕囊藻和绿色巴夫藻为研究对象,对金藻基因组DNA的提取方法进行了深入研究,并以psbA基因的PCR扩增结果对4种DNA提取方法进行了检测.结果显示,PVP法和高盐法安全、快速、经济,获得的基因组DNA产量高,但DNA杂质含量也较高.CTAB法虽然也具有安全、快速等优势,但提取的DNA产量较低.玻璃粉法提取的DNA质量好,产量也较高,但操作略为烦琐,经济费用高.结论:就球形棕囊藻而言,玻璃粉法是4种方法中最好的DNA提取方法.就绿色巴夫藻而言,4种方法都行,但是从经济和方便考虑,以高盐法为最好.

Genomic DNA extraction and PCR amplication of haptophytes

Phaeocystis globosa and Pavlova viridis which are colony and single cell representative were used as samples. Genomic DNA extraction from those haptophytes was studied. 4 methods for DNA extraction were examined, based on PCR amplification of psbA gene. The results showed that both salt-extraction of high concentration and PVP - extraction were safe, quick and economical methods. The high-yield DNA was extracted by the two methods, but impurity. The method of CTAB-extraction was also safe and quick, but the yield of genomic DNA was low. The glass bead-extraction can obtain high-yield and purity genomic DNA, but process is complex and expen- sive. For P. globosa, the method of glass bead-extraction was the best one among 4 methods. For P. viridis, considered economical and convenient factors, the method of salt-extraction of high was the best.