| 莱茵衣藻长链酰基辅酶A合成酶(LACS)同源基因的生物信息学分析 |
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| 引用本文： | 顾守来,马忠岩,谭小力.莱茵衣藻长链酰基辅酶A合成酶(LACS)同源基因的生物信息学分析[J].生物学杂志,2012,29(4):5-7,38. |
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| 作者姓名： | 顾守来 马忠岩 谭小力 |
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| 作者单位： | 江苏大学生命科学研究院,江苏,镇江,212013 |
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| 基金项目： | 国家重点基础研究发展计划(973) |
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| 摘 要： | 长链酰基辅酶A合成酶(LACS)能催化游离的脂肪酸形成酰基辅酶A硫脂,在油脂合成及降解途径中起着重要的作用.研究在莱茵衣藻(Chlamydomonas reinhardtii)中发现两个LACS基因,将其命名为CrLACS1和CrLACS2.生物信息学分析表明CrLACS1和CrLACS2在蛋白的理化性质及结构上都具有较高的相似性,而系统进化树分析显示CrLACS1和CrLACS2处于不同分枝,预测亚细胞定位也不相同.综合结果表明,CrLACS1和CrLACS2具有相似的结构,催化相同的反应,但具有不同的生物学功能.他们可能参与油脂代谢的不同途径:CrLACS1参与油脂的合成途径,而CrLACS2参与油脂的降解途径.
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| 关 键 词： | 莱茵衣藻 LACS 生物信息学 油脂 |
Bioinformatics analysis of long chain acyl-coenzyme A synthetases homologous genes in Chlamydomonas reinhardtii |
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| GU Shou-lai
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MA Zhong-yan
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TAN Xiao-li.Bioinformatics analysis of long chain acyl-coenzyme A synthetases homologous genes in Chlamydomonas reinhardtii[J].Journal of Biology,2012,29(4):5-7,38. |
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| Authors: | GU Shou-lai MA Zhong-yan TAN Xiao-li |
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| Institution: | (Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China) |
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| Abstract: | Long chain acyl-coenzyme A synthetases （LACSs） activate free fatty acid to acyl-CoA thioesters, and play important roles in the biosynthesis and degradation of lipids. In this study, two LACS genes were found in Chlamydomonas reinhardtii and designated as CrLACS1 and CrLACS2. CrLACS1 and CrLACS2 were highly similar in physicochemical characters and structure using bioinformatics methods. But, the phylogenetic tree analysis showed that CrLACS1 and CrLACS2 were in different clade, and subcellular localization analysis indicated that they were in different cellular organelles. Taken together, though CrLACS1 and CrLACS2 catalyzed the same reaction as their similar structures, they participated in the different pathways. CrLACS1 participated in the pathway of lipid biosynthesis, while CrLACS2 played a role in the degradation of lipid. |
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| Keywords: | Chlamydomonas reinhardtii LACS bioinformatics lipid |
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